Joe graduated with a degree in Genetics from the University of Leicester in 2008. During this period he also undertook a 12 month industrial placement with AstraZenecanc's Genetic support unit at Alderly Edge to develop DNA methylation analysis procedures. He followed this with a PhD at the University of Edinburgh, Wellcome Trust Centre for Cell Biology under the supervision of Dr Irina Stancheva. During his PhD Joe investigated the Somatic cell functions of Chromatin remodelling ATPase LSH. His epigenetic research interests were diverted when he revealed a novel role for LSH in DNA damage repair, work which was published in the Journal of Cell Biology in 2012. During his PhD Joe also participated in a number of industrial placements with CellCentric working on a range of projects including the orginisation of the 25 Years of Epigenetics conference at the University of Cambridge. Joe has now returned to Epigenetic research as a research technition to support the work of Professor Jonathan Mill's
epigenetic research group at the University of Exeter.
Cell culture techniques (cell lines):
- Viral packaging and infection
- Transfection
- Stable shRNA knockdown
- Soft agar assays
- Cell survival and viability studies
Molecular Biology:
- Nucleic acid extraction (DNA/RNA)
- Bisulphite treatment
- Whole genome amplification
- PCR / QPCR / Melt curve analysis
- Protein expression and purification
- Chromatin immunoprecipitation
- Western and Southern blotting
- Immunofluorescence
- Comet Assays
- Radiation handeling
Analysis Systems:
- Quiagen Pyrosequencing
- Illumina Infinium Methylation Assay
- LI-COR Bioscience Odyssey infrared imaging system
Burrage J, Termanis A, Geissner A, Myant K, Gordon K, Stancheva I. (2012). SNF2 family ATPase LSH promotes phosphorylation of H2AX and efficient repair of DNA double-strand breaks in mammalian cells. J Cell Sci. [Epub ahead of print]
Myant K, Termanis A, Sundaram AY, Boe T, Li C, Merusi C, Burrage J, de Las Heras JI, Stancheva I. (2011). LSH and G9a/GLP complex are required for developmentally programmed DNA methylation. Genome Res. 21, 83-94.
Knight LJ, Burrage J, Bujac SR, Haggerty C, Graham A, Gibson NJ, Ellison G, Growcott JW, Brooks AN, Hughes AM, Xinarianos G, Nikolaidis G, Field JK, Liloglou T. (2009). Epigenetic silencing of the endothelin-B receptor gene in non-small cell lung cancer. Int J Oncol. 34, 465-71.
